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Journal article
Whole-Genome sequencing of the schizomid arachnid Rowlandius potiguar from the caves of the Brazilian semiarid
Biodiversity genomes, Vol.2026
06/2026
PMID: 42487741
Abstract
Short-tailed whip scorpions (Arachnida: Schizomida) are a poorly studied group of arachnids, particularly in terms of genomic resources, despite their ecological and evolutionary relevance in subterranean environments. In this study, we present the first draft genome assembly of Rowlandius potiguar, a caveassociated species endemic to karst systems of northeastern Brazil. Genomic DNA was sequenced using Illumina paired-end technology (2 × 150 bp), and reads were assembled using a de novo assembly pipeline. The resulting genome assembly has a total length of 1.8 Gb, distributed across 1,505,104 scaffolds and 1,510,944 contigs, indicating a highly fragmented assembly. The scaffold N50 and contig N50 values were both 3.2 kb, and the GC content was 32.5%. Genome completeness was assessed using BUSCO with Arthropoda and Metazoa datasets. The Arthropoda analysis recovered 20.5% complete BUSCOs (19.1% single-copy and 1.5% duplicated), 53.0% fragmented, and 26.5% missing genes. Similarly, the Metazoa dataset identified 24.7% complete BUSCOs (23.4% single-copy and 1.4% duplicated), with 54.7% fragmented and 20.5% missing genes. The low BUSCO completeness and high proportion of fragmented genes reflect the highly fragmented nature of the assembly, likely resulting from the use of short-read sequencing technologies and the presence of repetitive genomic regions. Despite these limitations, this genome represents an important first genomic resource for Schizomida and provides a foundation for future studies on genome evolution, adaptation to subterranean environments, and the genetic basis of troglomorphic traits in arachnids.
Details
- Title
- Whole-Genome sequencing of the schizomid arachnid Rowlandius potiguar from the caves of the Brazilian semiarid
- Publication Details
- Biodiversity genomes, Vol.2026
- Resource Type
- Journal article
- Publisher
- Naturalis Historia Publishing
- Grant note
- Funding for sequencing was provided by Iridian Genomes (grant IRGEN_RG_2021-1345). LSM and OBD were supported by CAPES through PhD scholarships (LSM: #88887.483610/2020–00; OBD #88887.954829/2024-00). SMQL, MP, and RLF were supported by CNPq (SMQL #312066/2021–0, MP #310688/2019–1, RLF #302925/2022-8). This study was partially supported by the Speleological Compensation Commitment Agreements (TCCE 01/2018 and 01/2022), established between the Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio) and Vale S.A. Financial resources were managed by the Instituto Brasileiro de DEsenvolvimento e Sustentabilidade (IABS).
- Identifiers
- 99381839610206600
- Academic Unit
- Biology; Hal Marcus College of Science and Engineering
- Language
- English