Metrics
16 Record Views
Journal article
Using environmental DNA metabarcoding to assess the spatiotemporal occurrence of the imperiled River Redhorse (Moxostoma carinatum) in the Escambia-Conecuh River system of Florida and Alabama, USA
Environmental biology of fishes, Vol.107, pp.1593-1608
12/2024
Web of Science ID: WOS:001320221100001
Abstract
Environmental DNA (eDNA) metabarcoding is a novel molecular tool that is used to detect and catalog biodiversity from remnant DNA in the environment, including at-risk and elusive species. We used eDNA metabarcoding in this study to detect the spatiotemporal occurrence of River Redhorse (Moxostoma carinatum) and catalog the fish community in the Escambia-Conecuh River system of Florida and Alabama, hereafter referred to as the Escambia River system. The River Redhorse is a catostomid that has been classified as a Species of Greatest Conservation Need in Florida, as only three specimens have been collected from the Escambia-Conecuh River system in the last 70 years. In Alabama, only 20 specimens have been collected from this system since 2000. Surface water samples were collected at sites from the Escambia River system in December 2020 and March to June 2021. Universal MiFish primers were used and detected 47 fish species across sampled seasons from collected water samples, including River Redhorse. River Redhorse DNA was detected in high frequencies in all sampling seasons. The high occurrence of River Redhorse DNA across sampled localities provides evidence that the population is extant in the Escambia-Conecuh River system. Detections provide ideal spatiotemporal locations for conventional sampling methods to be deployed to gather essential biological data for the conservation and management of the River Redhorse. In addition, the metabarcoding approach cataloged the fish community temporally in the Escambia River System, indicating its usefulness as a complementary approach to conducting fish surveys with conventional sampling methods.
Related links
Details
- Title
- Using environmental DNA metabarcoding to assess the spatiotemporal occurrence of the imperiled River Redhorse (Moxostoma carinatum) in the Escambia-Conecuh River system of Florida and Alabama, USA
- Publication Details
- Environmental biology of fishes, Vol.107, pp.1593-1608
- Resource Type
- Journal article
- Publisher
- Springer
- Grant note
- This research was funded by the Kugelman Honors Program at the University of West Florida. Additional funding was provided by the Office of Undergraduate Research at the University of West Florida through undergraduate awarded grants and a Summer Undergraduate Research Program.
- Copyright
- © 2024, The Author(s), under exclusive licence to Springer Nature B.V.
- Identifiers
- WOS:001320221100001; 99380578756006600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Biology
- Language
- English