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Journal article
Use of qPCR to Monitor 2,4-Dinitroanisole Degrading Bacteria in Water and Soil Slurry Cultures
Journal of industrial microbiology & biotechnology
11/23/2024
PMID: 39580361
Web of Science ID: WOS:001373520900001
Abstract
Prediction and process monitoring during natural attenuation, bioremediation and biotreatment require effective strategies for detection and enumeration of the responsible bacteria. The use of 2,4-dinitroanisole (DNAN) as a component of insensitive munitions leads to environmental contamination of firing ranges and manufacturing waste streams. Nocardioides sp strain JS1661 degrades DNAN under aerobic conditions via a pathway involving an unusual DNAN demethylase. We used the deeply branched sequences of DNAN degradation functional genes as a target for development of a molecular method for detection of the bacteria. A qPCR assay was designed for the junction between dnhA and dnhB, the adjacent genes encoding DNAN demethylase. The assay allowed reproducible enumeration of JS1661 during growth in liquid media and soil slurries. Results were consistent with biodegradation of DNAN, accumulation of products and classical biomass estimates including most probable number and OD600. The results provide a sensitive and specific molecular method for prediction of degradation potential and process evaluation during degradation of DNAN.
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- Title
- Use of qPCR to Monitor 2,4-Dinitroanisole Degrading Bacteria in Water and Soil Slurry Cultures
- Publication Details
- Journal of industrial microbiology & biotechnology
- Resource Type
- Journal article
- Publisher
- Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology
- Format
- link
- Number of pages
- 27
- Copyright
- © The Author(s) 2024.
- Identifiers
- WOS:001373520900001; 99380589264206600
- Academic Unit
- Biology; Center for Environmental Diagnostics and Bioremediation ; Hal Marcus College of Science and Engineering
- Language
- English