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Journal article
Succinate transport by free-living forms of Rhizobium japonicum
Journal of bacteriology, Vol.153(3), pp.1155-1162
03/1983
PMID: 6402487
Web of Science ID: WOS:A1983QE72800005
Abstract
The authors have demonstrated that the transport of succinate into the cells of R. japonicum strains USDA 110 and USDA 217 is severely inhibited by cyanide, azide, and 2,4-dinitrophenol, but not by arsenate. These results suggest an active mechanism of transport that is dependent on an energized membrane, but does not directly utilize ATP. The expression of the succinate uptake activity was inducible rather than constitutive, with succinate and structurally related compounds being the most effective inducers. The mechansim showed some specificity for succinate and similar organic acids: fumarate and L-malate were classical competitive inhibitors of the system. In general, the best competing compounds were also the best carbon substrates for induction of succinate uptake activity. Growth media that were supplemented with increased Ca super(2+) concentration supported more rapid growth with succinate as the carbon substrate, and cells from such media showed higher specific activities of succinate transport.
Details
- Title
- Succinate transport by free-living forms of Rhizobium japonicum
- Publication Details
- Journal of bacteriology, Vol.153(3), pp.1155-1162
- Resource Type
- Journal article
- Publisher
- American Society for Microbiology
- Number of pages
- 8
- Copyright
- © 1983, American Society for Microbiology
- Identifiers
- WOS:A1983QE72800005; 99380592608606600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Biology; Center for Environmental Diagnostics and Bioremediation
- Language
- English