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Journal article
Revertible hydrogen uptake-deficient mutants of Rhizobium japonicum
Journal of bacteriology, Vol.146(2), pp.614-620
05/01/1981
PMID: 6783623
Web of Science ID: WOS:A1981LP48700021
Abstract
We have developed mutants of Rhizobium japonicum which are deficient in H2 uptake capacity (Hup-) and which spontaneously revert to the parent type at a frequency consistent with that of a single-point mutation (ca. 1.0 x 10(-09)). The mutagenesis by nitrous acid and the selection of the Hup- phenotype by using penicillin and chemolithotrophy as enrichment for chemolithotrophy-deficient strains are described. Two mutants retain low but reproducible levels of ribulose bisphosphate-dependent CO2 fixation when grown on a low-carbon medium under an atmosphere of 1% O2, 4% H2, 5% CO2, and 90% N2. Neither O2 nor the artificial electron acceptors phenazine methosulfate or methylene blue supported detectable H2 uptake by the free-living Hup- mutants or by their bacteroids. Plant growth experiments under bacteriologically controlled conditions were conducted to assess the mutants' performance as inocula for soybean plants. Plants inoculated with Hup- strains had lower dry weights and contained less total N than did plants inoculated with the parent Hup+ strain. Use of either the Hup- mutants or the Hup+ parent strain as inocula, however, did not significantly affect the acetylene-reducing activity or the fresh weight of nodules. These results, obtained with apparently isogenic lines of H2 uptake-deficient R. japonicum, provide strong support for a beneficial role of the H2 uptake phenotype in legume symbiosis.
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Details
- Title
- Revertible hydrogen uptake-deficient mutants of Rhizobium japonicum
- Publication Details
- Journal of bacteriology, Vol.146(2), pp.614-620
- Resource Type
- Journal article
- Number of pages
- 7
- Identifiers
- WOS:A1981LP48700021; 99380625065406600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Biology; Center for Environmental Diagnostics and Bioremediation
- Language
- English