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Journal article
PINK1 is dispensable for mitochondrial recruitment of Parkin and activation of mitophagy in cardiac myocytes
PL o S One, Vol.10(6)
10
2015
PMCID: PMC4482400
PMID: 26110811
Web of Science ID: WOS:000356933800075
Abstract
Myocyte function and survival relies on the maintenance of a healthy population of mitochondria. The PINK1/Parkin pathway plays an important role in clearing defective mitochondria via autophagy in cells. However, how the PINK1/Parkin pathway regulates mitochondrial quality control and whether it coordinates with other mitophagy pathways are still unclear. Therefore, the objective of this study was to investigate the effect of PINK1-deficiency on mitochondrial quality control in myocytes. Using PINK1-deficient (PINK1-/-) mice, we found that Parkin is recruited to damaged cardiac mitochondria in hearts after treatment with the mitochondrial uncoupler FCCP or after a myocardial infarction even in the absence of PINK1. Parkin recruitment to depolarized mitochondria correlates with increased ubiquitination of mitochondrial proteins and activation of mitophagy in PINK1-/- myocytes. In addition, induction of mitophagy by the atypical BH3-only protein BNIP3 is unaffected by lack of PINK1. Overall, these data suggest that Parkin recruitment to depolarized cardiac mitochondria and subsequent activation of mitophagy is independent of PINK1. Moreover, alternative mechanisms of Parkin activation and pathways of mitophagy remain functional in PINK1-/- myocytes and could compensate for the PINK1 deficiency.
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- Title
- PINK1 is dispensable for mitochondrial recruitment of Parkin and activation of mitophagy in cardiac myocytes
- Publication Details
- PL o S One, Vol.10(6)
- Resource Type
- Journal article
- Publisher
- Public Library of Science; United States
- Series
- 10
- Format
- pdf
- Copyright
- © 2015 Kubli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
- Identifiers
- WOS:000356933800075; 99380090298706600
- Academic Unit
- Movement Sciences and Health; Usha Kundu, MD College of Health
- Language
- English