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Journal article
Novel interactions between erythroblast macrophage protein and cell migration
Blood Cells, Molecules and Diseases, Vol.60, pp.24-27
60
2016
PMID: 27519940
Web of Science ID: WOS:000381702700007
Abstract
Erythroblast macrophage protein is a novel protein known to mediate attachment of erythroid cells to macrophages to form erythroblastic islands in bone marrow during erythropoiesis. Emp-null macrophages are small with round morphologies, and lack cytoplasmic projections which imply immature structure. The role of Emp in macrophage development and function is not fully elucidated.Macrophages performvaried functions (e.g. homeostasis, erythropoiesis), and are implicated in numerous pathophysiological conditions such as cellular malignancy. The objective of the current study is to investigate the interaction of Emp with cytoskeletal- and cell migration-associated proteins involved in macrophage functions. A short hairpin RNA lentiviral system was use to down-regulate the expression of Emp in macrophage cells. A cellmigration assay revealed that the relocation of macrophageswas significantly inhibitedwhen Emp expressionwas decreased. To further analyze changes in gene expression related to cellmotility, PCR arraywas performed by down-regulating Emp expression. The results indicated that expression of mitogen-activated protein kinase 1 and thymoma viral proto-oncogene 1 were significantly higher when Emp was down-regulated. The results implicate Emp in abnormal cell motility, thus, warrants to assess its role in cancer where tumor cell motility is required for invasion and metastasis.
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Details
- Title
- Novel interactions between erythroblast macrophage protein and cell migration
- Publication Details
- Blood Cells, Molecules and Diseases, Vol.60, pp.24-27
- Resource Type
- Journal article
- Publisher
- Academic Press; Orlando, FL
- Series
- 60
- Copyright
- 2016 Elsevier Inc.
- Identifiers
- WOS:000381702700007; 99380090645106600
- Academic Unit
- Usha Kundu, MD College of Health; Movement Sciences and Health
- Language
- English