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Journal article
Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency
Acta physiologiae plantarum, Vol.37(2), 42
02/2015
Web of Science ID: WOS:000350050100042
Abstract
Nitrate (NO3-) can accumulate in high concentrations in plant cell vacuoles if it is not reduced, reutilized or transported into the cytoplasm. Such accumulation of NO3- in the vacuole occurs when mechanisms for NO3- assimilation in the cytoplasm are saturated. Moreover, other processes such as efflux across the plasma membrane might affect NO3- accumulation in the vacuole. These are the main reasons limiting nitrogen use efficiency (NUE) in plants. This study elucidates mechanisms for NO3- transport from the cytoplasm to vacuoles by the V-proton pump (V-ATPase and V-PPase) and their relationship with different NUE in four Brassica napus genotypes. Pot experiments were conducted in a greenhouse under normal (15.0 mmol L-1) and limited N (7.5 mmol L-1) concentrations of nitrate using B. napus genotypes that demonstrated either high (742 and Xiangyou 15) or low (814 and H8) NUE (g g(-1)). Specific inhibitors of V-ATPase and V-PPase increased nitrate reductase (NR) activity, resulting in greatly decreased NO3- in plant tissues. Nitrate reductase activity and NO3- content correlated more highly to V-PPase activity than they did to V-ATPase activity, and correlation between V-PPase activity and NO3- content was significantly higher than it was to V-ATPase. Genotypes with high NUE had significantly lower activities of V-ATPase and V-PPase than those with low NUE. In the high-NUE plants, lower activities of V-proton pump underlie mechanisms that result in significantly lower NO3- content in plant tissues of the high-NUE genotypes than those found in plant tissues of the low-NUE genotypes. Our results show that the tonoplast proton pumps V-PPase and V-ATPase strongly negatively affect NR activity and positively affect NO3- content. V-PPase contributed more to this regulatory mechanism than did V-ATPase.
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Details
- Title
- Nitrate reutilization mechanisms in the tonoplast of two Brassica napus genotypes with different nitrogen use efficiency
- Publication Details
- Acta physiologiae plantarum, Vol.37(2), 42
- Resource Type
- Journal article
- Publisher
- Springer Nature
- Number of pages
- 13
- Grant note
- 2012BAD15BO4 / National Key Laboratory of Plant Molecular Genetics, The "Twelfth Five-Year" National Science and technology support program 13K062 / Open novel science foundation of Hunan province 31101596; 31372130 / National Natural Science Foundation of China; National Natural Science Foundation of China (NSFC)
- Copyright
- © 2015, Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków
- Identifiers
- WOS:000350050100042; 99380592695406600
- Academic Unit
- Biology; Center for Environmental Diagnostics and Bioremediation ; Hal Marcus College of Science and Engineering
- Language
- English