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Journal article
Muscle cytokine mRNA changes after 2.5 h of cycling: Influence of carbohydrate
Medicine and science in sports and exercise, Vol.37(8), pp.1283-1290
08/01/2005
PMID: 16118573
Web of Science ID: WOS:000231476000005
Abstract
Purpose:
To study the effect of carbohydrate compared to placebo ingestion on plasma cytokines and muscle cytokine mRNA following 2.5 h of intensive cycling in 15 trained cyclists.
Methods:
Fifteen trained cyclists cycled for 2.5 h at 60% Wmax on two occasions while receiving 4 mL·kg−1·15 min−1 carbohydrate (6%) (CHO) or placebo (PLA) beverages in a randomized, counterbalanced design. Blood and vastus lateralis muscle biopsy samples were collected before and after exercise and 12 h post-exercise and compared to samples taken from five cyclists who rested in the lab during the exercise sessions. Blood cell counts were determined, and plasma was analyzed for interleukin (IL)-6, IL-10, IL-1 receptor antagonist (ra), IL-8, cortisol, epinephrine, glucose, and insulin. Muscle was analyzed for glycogen content and relative gene expression of four cytokines, IL-6, IL-8, tumor necrosis factor (TNF) α, and IL-1β, using real-time quantitative reverse transcriptase polymerase chain reaction.
Results:
Plasma glucose and insulin were higher, and epinephrine, cortisol, IL-6, IL-10, and IL-1ra, but not IL-8, were significantly lower pos-texercise in CHO versus PLA. Muscle glycogen content decreased 68% immediately post-exercise and the pattern of change did not differ between CHO and PLA. Muscle IL-6, IL-8, TNF-α, but not IL-1β mRNA increased immediately post-exercise compared to controls, with no differences between CHO and PLA.
Conclusion:
CHO compared to PLA beverage ingestion attenuated the increase in plasma cortisol, epinephrine, IL-6, IL-10, and IL-1ra, but not muscle IL-6, IL-8, and TNF-α mRNA in athletes cycling 2.5 h at 60% Wmax.
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Details
- Title
- Muscle cytokine mRNA changes after 2.5 h of cycling
- Publication Details
- Medicine and science in sports and exercise, Vol.37(8), pp.1283-1290
- Resource Type
- Journal article
- Publisher
- Lippincott Williams & Wilkins
- Grant note
- Supported by a grant from the Gatorade Sports Science Institute
- Copyright
- © 2005, ©2005The American College of Sports Medicine
- Identifiers
- WOS:000231476000005; 99380186196306600
- Academic Unit
- Movement Sciences and Health; Usha Kundu, MD College of Health
- Language
- English