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Journal article
Measurement of diameters of estuarine bacteria and particulates in natural water samples by use of a submicron particle analyzer
Current microbiology, Vol.10(1), pp.7-11
01/1984
Web of Science ID: WOS:A1984SD37600002
Abstract
Diffusion coefficients and mean diameters of estuarine bacterial isolates and particulates from Bayboro Harbor water, St. Petersburg, FL, were measured by photon correlation spectroscopy (PCS) employing a Coulter N4 Submicron Particle Analyzer. Mean diameters of formalin-killed bacteria by PCS agreed with those obtained from Nomarski Interference and epifluorescence microscopy. Diffusion coefficients of motile bacteria were 1.3–4.4 times greater than for formalin-killed bacteria, and PCS-estimated diameters were accordingly smaller. Analysis of Bayboro Harbor water by PCS indicated a highly variable distribution of particle diameters in unfiltered water samples. Filtration through 3-, 1-, 0.4-, and 0.2-μm Nucleopore filters resulted in progressively smaller, reproducible particle diameter distributions, which again agreed with measurements made by epifluorescence microscopy.
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Details
- Title
- Measurement of diameters of estuarine bacteria and particulates in natural water samples by use of a submicron particle analyzer
- Publication Details
- Current microbiology, Vol.10(1), pp.7-11
- Resource Type
- Journal article
- Publisher
- Springer New York LLC
- Copyright
- © 1969, Springer-Verlag
- Identifiers
- WOS:A1984SD37600002; 99380184795006600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Center for Environmental Diagnostics and Bioremediation
- Language
- English