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Journal article
Kit-On-A-Lid-Assays for accessible self-contained cell assays
Lab on a chip, Vol.13(3), pp.424-431
02/07/2013
PMCID: PMC3562598
PMID: 23229806
Web of Science ID: WOS:000312947300014
Abstract
Microscale methods for cell-based assays typically rely on macroscopic reagent handling and fluidic loading protocols that are technically challenging and do not scale with the number of assays favorably. Here, we demonstrate a microfluidic platform technology called "Kit-On-A-Lid-Assay" ( KOALA), that enables the creation of self-contained microfluidic cell-based assays, integrating all the steps required to perform cell-based assays. The KOALA platform allows the pre-packaging of reagents, cryopreservation of cell suspensions, thawing of cell suspensions, culture of cells, and operation of whole cell-based assays. The operation of the KOALA platform is user-friendly and consists of bringing together a lid containing the microchannels, and a base containing the pre-packaged reagents, thereby causing fluidic exchange in all the channels simultaneously. We demonstrate that the KOALA cell-based assays can be simply operated from start to finish without any external laboratory equipment.
Details
- Title
- Kit-On-A-Lid-Assays for accessible self-contained cell assays
- Publication Details
- Lab on a chip, Vol.13(3), pp.424-431
- Resource Type
- Journal article
- Publisher
- Royal Soc Chemistry
- Format
- pdf
- Number of pages
- 8
- Grant note
- R01 EB010039 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA T32ES007015 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS) Bill and Melinda Gates Foundation through the Grand Challenges in Global Health Initiative; Bill & Melinda Gates Foundation National Science Foundation; National Science Foundation (NSF) American Heart Association R01EB010039 / NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Biomedical Imaging & Bioengineering (NIBIB)
- Identifiers
- WOS:000312947300014; 99380171296406600
- Academic Unit
- Biology; Hal Marcus College of Science and Engineering
- Language
- English