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Journal article
Key Glycolytic Metabolites in Paralyzed Skeletal Muscle Are Altered Seven Days after Spinal Cord Injury in Mice
Journal of neurotrauma, Vol.36(18), pp.2722-2731
09/15/2019
PMID: 30869558
Web of Science ID: WOS:000464545800001
Abstract
Spinal cord injury (SCI) results in rapid muscle atrophy and an oxidative-to-glycolytic fiber-type shift. Those with chronic SCI are more at risk for developing insulin resistance and reductions in glucose clearance than able-bodied individuals, but how glucose metabolism is affected after SCI is not well known. An untargeted metabolomics approach was utilized to investigate changes in whole-muscle metabolites at an acute (7-day) and subacute (28-day) time frame after a complete T9 spinal cord transection in 20-week-old female C57BL/6 mice. Two hundred one metabolites were detected in all samples, and 83 had BinBase IDs. A principal components analysis showed the 7-day group as a unique cluster. Further, 36 metabolites were altered after 7- and/or 28-day post-SCI (p values <0.05), with 12 passing further false discovery rate exclusion criteria; of those 12 metabolites, three important glycolytic molecules-glucose and downstream metabolites pyruvic acid and lactic acid-were reduced at 7 days compared to those values in sham and/or 28-day animals. These changes were associated with altered expression of proteins associated with glycolysis, as well as monocarboxylate transporter 4 gene expression. Taken together, our data suggest an acute disruption of skeletal muscle glucose uptake at 7 days post-SCI, which leads to reduced pyruvate and lactate levels. These levels recover by 28 days post-SCI, but a reduction in pyruvate dehydrogenase protein expression at 28 days post-SCI implies disruption in downstream oxidation of glucose.
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Details
- Title
- Key Glycolytic Metabolites in Paralyzed Skeletal Muscle Are Altered Seven Days after Spinal Cord Injury in Mice
- Publication Details
- Journal of neurotrauma, Vol.36(18), pp.2722-2731
- Resource Type
- Journal article
- Publisher
- Mary Ann Liebert, Inc
- Number of pages
- 10
- Grant note
- 1U24DK097154 / NIH grant; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA Department of Veterans Affairs Rehabilitation Research& Development Service's National Center for the Medical Consequences of Spinal Cord Injury (B2020C); US Department of Veterans Affairs
- Copyright
- © 2019, Mary Ann Liebert, Inc.
- Identifiers
- WOS:000464545800001; 99380570796706600
- Academic Unit
- Biology; Hal Marcus College of Science and Engineering
- Language
- English