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Journal article
Investigating the Epigenetic Effects of Polystyrene Nanoplastic Exposure in Bluegill (Lepomis macrochirus) Epithelial Cells Using Methylation-Sensitive AFLPs
Microplastics, Vol.4(1), p.10
02/27/2025
Web of Science ID: WOS:001482365900001
Abstract
Microplastics, remnants of macroplastics that have broken down to fragments smaller than 5 mm, and nanoplastics, broken down even further to sizes < 1 μm, are pervasive in aquatic ecosystems. These plastic particles are consumed by microscopic organisms, leading to bioaccumulation up trophic levels. The accumulation of plastic in the organismal gut can result in various repercussions, including cellular contamination and genomic modifications such as DNA methylation. While methylation has been studied in teleost fishes, the impact of nanoplastic exposure on this process in any species remains largely unexplored. This study delves into this largely uncharted territory, investigating the accumulation of methylation due to nanoplastic exposure within the genome of cultured bluegill BF-2 cells (Lepomis macrochirus) using methylation-sensitive AFLPs. The methylation state was analyzed through capillary gel analysis and electropherograms. Differential methylation occurred between several control and experimental groups due to nanoplastic exposure; however, these differences were not dose- or time-dependent. These results could suggest that higher dosages and exposure times to nanoplastics do not result in increased methylation levels in congruence with the dosage and exposure time; rather, only the presence of nanoplastics is enough to cause DNA methylation changes.
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- Title
- Investigating the Epigenetic Effects of Polystyrene Nanoplastic Exposure in Bluegill (Lepomis macrochirus) Epithelial Cells Using Methylation-Sensitive AFLPs
- Publication Details
- Microplastics, Vol.4(1), p.10
- Resource Type
- Journal article
- Publisher
- MDPI; BASEL
- Number of pages
- 18
- Grant note
- University of West Florida Hal Marcus College of Science and Engineering Graduate GrantUniversity of West Florida Hal Marcus College of Science and Engineering Graduate Grant, University of West Florida Biology Department
This research was funded by the University of West Florida Hal Marcus College of Science and Engineering Graduate Grant, University of West Florida Biology Department.
- Copyright
- © 2025 by the authors.
- Identifiers
- WOS:001482365900001; 99381305835206600
- Academic Unit
- Biology; Hal Marcus College of Science and Engineering
- Language
- English