Journal article
Integrin associated proteins differentially regulate neutrophil polarity and directed migration in 2D and 3D
Biomedical microdevices, Vol.17(5), pp.100-100
10/01/2015
PMCID: PMC4678772
PMID: 26354879
Web of Science ID: WOS:000362281200017
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Abstract
Directed neutrophil migration in blood vessels and tissues is critical for proper immune function; however, the mechanisms that regulate three-dimensional neutrophil chemotaxis remain unclear. It has been shown that integrins are dispensable for interstitial three-dimensional (3D) leukocyte migration; however, the role of integrin regulatory proteins during directed neutrophil migration is not known. Using a novel microfluidic gradient generator amenable to 2D and 3D analysis, we found that the integrin regulatory proteins Kindlin-3, RIAM, and talin-1 differentially regulate neutrophil polarization and directed migration to gradients of chemoattractant in 2D
versus
3D. Both talin-1-deficient and RIAM-deficient neutrophil-like cells had impaired adhesion, polarization, and migration on 2D surfaces whereas in 3D the cells polarized but had impaired 3D chemotactic velocity. Kindlin-3 deficient cells were able to polarize and migrate on 2D surfaces but had impaired directionality. In a 3D environment, Kindlin-3 deficient cells displayed efficient chemotaxis. These findings demonstrate that the role of integrin regulatory proteins in cell polarity and directed migration can be different in 2D and 3D.
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Details
- Title
- Integrin associated proteins differentially regulate neutrophil polarity and directed migration in 2D and 3D
- Publication Details
- Biomedical microdevices, Vol.17(5), pp.100-100
- Resource Type
- Journal article
- Copyright
- © 2015, Springer Science Business Media New York
- Identifiers
- WOS:000362281200017; 99380171097106600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Biology
- Language
- English