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Inactivation of Human Immunodeficiency Virus by a Medical Waste Disposal Process Using Chlorine Dioxide
Journal article   Peer reviewed

Inactivation of Human Immunodeficiency Virus by a Medical Waste Disposal Process Using Chlorine Dioxide

Robert W Farr and Cheryl Walton
Infection control and hospital epidemiology, Vol.14(9), pp.527-529
09/1993
DOI: https://doi.org/10.1086/646798
PMID: 8228160
Web of Science ID: WOS:A1993LW97400008

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Abstract

Objective: To study the ability of a medical waste disposal process using chlorine dioxide to inactivate human immunodeficiency virus type 1 (HIV-1). Design: Stock HIV-1 (HTLV-IIIB strain) was treated with chlorine dioxide under the following settings: cell culture medium alone, culture medium with 25% blood, culture medium with medical supplies treated by the Condor machine (Winfield Environmental Corp., Escondido, CA). MT-2 cells in 96-well tissue culture plates were inoculated with serial tenfold dilutions of treated and untreated HIV-1. Cytopathic effect was read on day five, and the$TCID_{50}$(50% tissue culture infectious dose) was calculated. Results: Treatment of HIV-1 with chlorine dioxide in culture medium alone resulted in a 5.25$log_{10}$reduction in$TCID_{50}$. Treatment of HIV-1 with chlorine dioxide in the presence of 25% blood caused a 6.25$log_{10}$reduction in HIV-1 infectivity. Treatment of HIV-1 with chlorine dioxide in the presence of medical supplies treated in the Condor machine resulted in a 4.75$log_{10}$reduction in HIV infectivity. Conclusions: Chlorine dioxide inactivated HIV-1 in vitro. Chlorine dioxide inactivated HIV-1 in the presence of blood and in the presence of medical supplies under conditions that simulated the conditions existing in the Condor machine.

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