Genomic heterogeneity in pancreatic cancer organoids and its stability with culture

The authors would like to thank David A. Tuveson (Cold Spring Harbor Laboratory) and Herve Tiriac (University of California San Diego) for their support and guidance in PDAC organoid culture. The authors would like to thank Dr. Terra Bradley for the careful editing of the manuscript. The authors would like to thank Drs. Cynthia Vied and Yanming Yang at Florida State University's Translational Science Laboratory for running the scWGS and RNA-seq libraries and Drs. Yong Liu and Beth Alexander at Florida State University's Confocal Microscopy Laboratory. The authors would like to also thank Dr. Maria L Lemma at the Center for Applied Genomics at the Children's Hospital of Philadelphia for the SNP microarray. The authors in this study were supported by startup funds from Florida State University, an award from the Florida Department of Health's Bankhead-Coley Cancer Research Program (award number 21B11), and a collaborative Trans-Network Project linked to the Physical Science-Oncology Project 5U01 CA214282 from the National Cancer Institute of the National Institutes of Health. The hPT1 PDAC organoid line is from the Barts Pancreas Tissue Bank, a research tissue bank at the Barts Cancer Institute, Queen Mary University of London, London, UK, https://www.bartspancreastissuebank.org.uk/index.html, and funded by Pancreatic Cancer Research Fund. Ahmet Imrali was instrumental in developing the organoids at the Barts Pancreas Tissue Bank. The hPT2 PDAC organoid line is from the Human Cancer Models Initiative, https://ocg.cancer.gov/programs/HCMI.