Characterizing organisms from three domains of life with universal primers from throughout the global ocean

We would like to acknowledge the help of Laura Furtado (Fuhrman Lab Technician) for her assistance and support with lab work, sequencing, and organizing the postage of samples. We would also like to acknowledge the help of Bruce Yanpui Chan, as well as Yi-Chun Yeh for their support with DNA extractions, DNA amplification and sequencing. We would like to acknowledge Bror F Jonsson for providing the longhurst allocation scripts and predicted euphotic depths. We also acknowledge the Center for Advanced Research Computing (CARC) at the University of Southern California for providing computing resources that have contributed to the research results reported within this publication. URL: https://carc.usc.edu.We would like to acknowledge the help of many crew and technicians of various cruises on which data for this project were collected. For AMT, we would like to thank the captain and crew of the RRS James Cook, Stephanie Sargeant, and the marine technicians from UK National Marine Facilities for their assistance in collecting these samples. For FRAM and MOSAiC, we would like to thank the captain and crew of RV Polarstern expeditions PS99.2 and PS107, as well as the FRAM/HAUSGARTEN team, for their exceptional support. We thank Jana Baeger, Theresa Hargesheimer, Rafael Stiens, Lili Hufnagel, Daniel Scholz, Normen Lochthofen, Janine Ludszuweit, Lennard Frommhold and Jonas Hagemann for RAS and mooring operation; Jakob Barz and Swantje Ziemann for DNA extraction and library preparation; Halina Tegetmeyer for amplicon sequencing; and Laura Wischnewski for nutrient analyses. For BioGEOTRACES we would like to thank the captain and crew of the R/V Pelagia, R/V Southern Surveyor, R/V Tangaroa, RRS James Cook, RRS Discovery, and R/V Knorr, and Kristin Bergauer, Heather A. Bouman, Thomas J. Browning, Daniele De Corte, Christel Hassler, Debbie Hulston, Jeremy E. Jacquot, Elizabeth W. Maas, Thomas Reinthaler, Eva Sintes, and Taichi Yokokawa for their assistance in collecting these samples. For GRADIENTS we would like to thank the captain and crew of the R/V Marcus G. Langseth (2017) and R/V Kilo Moana (2019) for their assistance in collecting these samples, as well as Chief Scientist Ginger Armbrust. For the Southern Oceans HEOBI cruise we acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) in undertaking this research. We would like to thank the captain and crew of the RV Investigator, and the support of an Antarctic Science International Bursary awarded to Swan LS Sow (University of Tasmania) for their assistance in collecting these samples. For the P15S cruise we acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) in undertaking this research. We would like to thank the captain and crew of the RV Investigator for their assistance in collecting these samples. For the INS2017 cruise we acknowledge the use of the CSIRO Marine National Facility (https://ror.org/01mae9353) in undertaking this research. We would like to thank the captain and crew of the RV Investigator and for their assistance in collecting these samples and the funding support of an Antarctic Science International Bursary awarded to Swan LS Sow (University of Tasmania). For the KAXIS cruise we would like to thank the captain and crew of the RV Aurora Australis and the Science Technical Support Team of the Australian Antarctic Division for their assistance in collecting these samples and the funding support of an Antarctic Science International Bursary awarded to Swan LS Sow (University of Tasmania). For the I9N and I8S cruises we would like to thank the captain and crew of the R/V Roger Revelle, UCSB at-sea team members Chantal Swan and Dave Menzies and especially the trace metals teams from U.Hawaii (Chris Measures Lab) and Florida State Univ. (Bill Landing lab) for their assistance in collecting these samples. For both P16N and P16S we would like to thank the captain and crew of the R/V Thomas G. Thompson and R/V Roger Revelle for their assistance in collecting these samples, as well as Chief Scientists Christopher L. Sabine (P16N), Richard A. Feely (P16N), and Bernadette M. Sloyan (P16S). Finally, for POTATOE, we would like to thank the captain and crew of the RVIB Nathaniel B Palmer for their assistance in collecting these samples. This project was funded by the Simons Collaboration on Computational Biogeochemical Modeling of Marine Ecosystems (CBIOMES) Award #549943 and the National Science Foundation (NSF) grant EF-2125142.