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Journal article
Balance between nitrogen use efficiency and cadmium tolerance in Brassica napus and Arabidopsis thaliana
Plant science (Limerick), Vol.284, pp.57-66
07/2019
PMID: 31084879
Web of Science ID: WOS:000470042100008
Abstract
•Reduced V-ATPase and V-PPase activity improved nitrogen use efficiency.
•Reduced V-ATPase and V-PPase activity decreased Cd2+ tolerance.•Decreased NO3− vacuolar sequestration capacity (VSC) enhanced Atclca-2 Cd2+ VSC.
•Enhanced Cd2+ VSC decreased NO3− VSC in AtCAX4-OE.•Regulating Cd2+ and NO3− vacuolar accumulation enhances NUE and Cd2+ tolerance.
The transmembrane transport of NO3− and Cd2+ into plant cell vacuoles relies on the energy from their tonoplast proton pumps, V-ATPase and V-PPase. If the activity of these pumps is reduced, it results in less NO3− and Cd2+ being transported into the vacuoles, which contributes to better nitrogen use efficiency (NUE) and lower Cd2+ tolerance in plants. The physiological mechanisms that regulate the balance between NUE and Cd2+ tolerance remain unknown. In our study, two Brassica napus genotypes with differential NUEs, xiangyou 15 and 814, and Atclca-2 mutant and AtCAX4 over-expression line (AtCAX4-OE) of Arabidopsis thaliana, were used to investigate Cd2+ stress responses. We found that the Brassica napus genotype, with higher NUE, was more sensitive to Cd2+ stress. The AtCAX4-OE mutant, with higher Cd2+ vacuolar sequestration capacity (VSC), limited NO3− sequestration into root vacuoles and promoted NUE. Atclca-2 mutants, with decreased NO3− VSC, enhanced Cd2+ sequestration into root vacuoles and conferred greater Cd2+ tolerance than the WT. This may be due to the competition between Cd2+ andNO3− in the vacuoles for the energy provided by V-ATPase and V-PPase. Regulating the balance between Cd2+ and NO3− vacuolar accumulation by inhibiting the activity of CLCa transporter and increasing the activity of CAX4 transporter will simultaneously enhance both the NUE and Cd2+ tolerance of Brassica napus, essential for improving its Cd2+ phytoremediation potential.
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Details
- Title
- Balance between nitrogen use efficiency and cadmium tolerance in Brassica napus and Arabidopsis thaliana
- Publication Details
- Plant science (Limerick), Vol.284, pp.57-66
- Resource Type
- Journal article
- Publisher
- Elsevier B.V
- Number of pages
- 10
- Copyright
- © 2019 Elsevier B.V. All rights reserved.
- Identifiers
- WOS:000470042100008; 99380592695506600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Biology; Center for Environmental Diagnostics and Bioremediation
- Language
- English