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Journal article
Bacterial Survival under Extreme UV Radiation: A Comparative Proteomics Study of Rhodobacter sp., Isolated from High Altitude Wetlands in Chile
Frontiers in microbiology, Vol.8, pp.1173-1173
06/26/2017
PMCID: PMC5483449
PMID: 28694800
Web of Science ID: WOS:000404006000001
Abstract
Salar de Huasco, defined as a polyextreme environment, is a high altitude saline wetland in the Chilean Altiplano (3800 m.a.s.l.), permanently exposed to the highest solar radiation doses registered in the world. We present here the first comparative proteomics study of a photoheterotrophic bacterium,
Rhodobacter
sp., isolated from this remote and hostile habitat. We developed an innovative experimental approach using different sources of radiation (
in situ
sunlight and UVB lamps), cut-off filters (Mylar, Lee filters) and a high-throughput, label-free quantitative proteomics method to comprehensively analyze the effect of seven spectral bands on protein regulation. A hierarchical cluster analysis of 40 common proteins revealed that all conditions containing the most damaging UVB radiation induced similar pattern of protein regulation compared with UVA and visible light spectral bands. Moreover, it appeared that the cellular adaptation of
Rhodobacter
sp. to osmotic stress encountered in the hypersaline environment from which it was originally isolated, might further a higher resistance to damaging UV radiation. Indeed, proteins involved in the synthesis and transport of key osmoprotectants, such as glycine betaine and inositol, were found in very high abundance under UV radiation compared to the dark control, suggesting the function of osmolytes as efficient reactive oxygen scavengers. Our study also revealed a RecA-independent response and a tightly regulated network of protein quality control involving proteases and chaperones to selectively degrade misfolded and/or damaged proteins.
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- Title
- Bacterial Survival under Extreme UV Radiation
- Publication Details
- Frontiers in microbiology, Vol.8, pp.1173-1173
- Resource Type
- Journal article
- Publisher
- Frontiers Media S.A
- Format
- link
- Grant note
- This research was supported by grants Fondecyt N°1140179, N°1140356, and CeBiB FB0001. VP is the recipient of a Conicyt Ph.D. fellowship. Open access was funded by the University of Stirling.
- Identifiers
- WOS:000404006000001; 99380184621606600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Center for Environmental Diagnostics and Bioremediation
- Language
- English