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Journal article
A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR
Journal of microbiological methods, Vol.89(1), pp.76-78
04/2012
PMID: 22314021
Web of Science ID: WOS:000302760700014
Abstract
Propidium monoazide (PMA) was used to differentiate live from membrane-compromised bacteria in PCR methods. We have adapted this technique for use on membrane-filtered water samples and determined its efficacy using qPCR. Independent labs at three institutions replicated these findings.
► PMA differentiation of live-dead PCR targets adapted to membrane-filtered waters.
► An array of 16 blue LEDs replaced quartz-halogen activation of PMA on bacteriological filters.
► We compared qPCR Ct of DNA from “live” and “dead” filtered samples.
► PMA treatment inhibited PCR of bacterial DNA from "dead” but not “live” samples.
► Similar results were obtained at three independent laboratories.
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Details
- Title
- A filter-based propidium monoazide technique to distinguish live from membrane-compromised microorganisms using quantitative PCR
- Publication Details
- Journal of microbiological methods, Vol.89(1), pp.76-78
- Resource Type
- Journal article
- Publisher
- Elsevier B.V
- Number of pages
- 3
- Grant note
- This research was supported by US EPA for Gulf of Mexico Alliance Regional Partnership Projects MX-96478707-0. Additional support from the UWF Office of Research and Sponsored projects was provided to JEL and a UWF Scholarly and Creative Activities grant was provided to EMK.
- Copyright
- © 2012 Elsevier B.V. All rights reserved.
- Identifiers
- WOS:000302760700014; 99380592695006600
- Academic Unit
- Hal Marcus College of Science and Engineering ; Biology; Center for Environmental Diagnostics and Bioremediation
- Language
- English