Metrics
1 File views/ downloads
85 Record Views
Thesis
marine pinfish, Lagodon rhomboides, as a model for developmental neurogenesis and retinal development
University of West Florida,
Master of Science (MS), University of West Florida
2019
Abstract
In contrast to mammals, the fish CNS produces new neurons throughout life and, following injury, neurons fully regenerate and function is restored. Understanding how and why persistent neurogenesis and CNS regeneration occur in fish, but not in mammals, is crucial for developing the ability to treat CNS injury and disease in humans. The zebrafish has a relatively simple early developmental period compared with many marine fish species and does not represent the full diversity of developmental mechanisms among vertebrates. Therefore, our objective is to develop the pinfish, Lagodon rhomboides, as a marine fish laboratory model that will improve our understanding of the complex factors that affect retinal neurogenesis. In early experiments, the Rhodopsin gene, a critical visual pigment in rod photoreceptors, was cloned from the pinfish retina and sequenced. Next generation sequencing was used to sequence the entire pinfish genome, which will be gradually assembled. Additionally, pinfish have been maintained in fiberglass tanks equipped to control light cycle, temperature, and other environmental factors that usually induce spawning in marine fish. Manipulating these factors to simulate the ideal conditions for the spawning will allow this project to be carried out throughout the year. Spawning has been induced, and larvae have been raised up to metamorphosis. Early data suggest that salinity may influence the timing of metamorphosis. In lower salinity, the onset of metamorphosis was observed at 9 days. However, in higher salinity, metamorphosis was not observed up to 17 days post hatch. The next step is to determine whether salinity affects timing xi of metamorphosis. Subsequently, we will determine the timing of photoreceptor genesis during development.
Details
- Title
- marine pinfish, Lagodon rhomboides, as a model for developmental neurogenesis and retinal development
- Resource Type
- Thesis
- Publisher
- University of West Florida,; Pensacola, Florida :
- Format
- text;electronic resource;remote;computer;online resource
- Number of pages
- 1 online resource (xi, 49 leaves : illustrations, charts)
- Copyright
- http://rightsstatements.org/vocab/InC-EDU/1.0/
- Identifiers
- 99380090857406600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida