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Thesis
THE EFFECTS OF LITHIUM AND VALPROIC ACID ON THE PHENOTYPIC STATE, PHOSPHORYLATED PROTEOME AND CALCIUM-BINDING PROTEOME OF TETRAHYMENA PATULA
University of West Florida
Master of Science (MS), University of West Florida
2011
Abstract
The effects of lithium and valproic acid on the phenotypic state of Tetrahymena patula were investigated. As related to the effects on phenotype, the phosphorylated proteome and calcium-binding proteome of T. patula were examined for changes in order to gain insight into the biochemical targets of lithium and valproic acid. Samples taken from various treatments were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. Gels were stained with Pro-Q Diamond stain for phosphoproteins and Sypro Ruby stain for total proteins in order to discern changes in degree of treatmentdependent protein phosphorylation. Changes were digitally quantified. Separate gels were also stained to visualize any qualitative change in the presence of calcium-binding proteins. Patterns of treatment-dependent morphological changes correlated to changes with an increase in the degree of phosphorylation of a 59 kDa protein and 43 kDa protein. These changes may implicate the involvement of phosphoinositide signaling in the dedifferentiation of T. patula. The changes in the presence of 60 kDa and 115 kDa calcium-binding proteins appeared to be treatment-dependant and related to morphological change.
Details
- Title
- THE EFFECTS OF LITHIUM AND VALPROIC ACID ON THE PHENOTYPIC STATE, PHOSPHORYLATED PROTEOME AND CALCIUM-BINDING PROTEOME OF TETRAHYMENA PATULA
- Resource Type
- Thesis
- Publisher
- University of West Florida
- Format
- text
- Identifiers
- 99380090868306600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida