Thesis
THE EFFECTS OF ACUTE, MODERATE-INTENSITY ENDURANCE EXERCISE ON MITOCHONDRIAL BIOGENESIS IN NEUTROPHILS
University of West Florida
Master of Science (MS), University of West Florida
2014
Abstract
This study tested the hypothesis that moderate-intensity endurance exercise would induce mitochondrial biogenesis in the human neutrophil. Nine healthy, recreationally-active males (ages 20-27) performed 30 min of moderate-intensity (50% of VO2max) cycling exercise for two consecutive days. Blood samples were drawn on three separate time points (pre exercise, post exercise, and one hour after exercise). Neutrophils were isolated from the whole blood samples and treated and prepped for western blotting analysis. Mitochondrial transcription factor A and mitochondrial complex proteins were not detected by western blotting, and NRF-1 was detected for only three of the subjects. Friedman's two-way ANOVA was performed on levels of PGC-1a and NRF-1, and no significance was established (p = 0.175 and p = 0.544, respectively).
Details
- Title
- THE EFFECTS OF ACUTE, MODERATE-INTENSITY ENDURANCE EXERCISE ON MITOCHONDRIAL BIOGENESIS IN NEUTROPHILS
- Resource Type
- Thesis
- Publisher
- University of West Florida
- Format
- text
- Identifiers
- 99380090750906600
- Academic Unit
- Usha Kundu, MD College of Health
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida