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Thesis
Regrowth of Subtropical Aerobic Anoxygenic Photoheterotrophic Bacteria Upon Grazer Removal: A Case Study of Surface Waters Collected from the Southern Pensacola Bay
University of West Florida Libraries
Master of Science (MS), University of West Florida
Summer 2022
Abstract
Aerobic anoxygenic photoheterotrophic bacteria (AAPBs) are heterotrophic bacteria capable of harvesting light energy using bacteriochlorophyll a bound to the reaction center. This reaction center, comprised of various proteins, one of which is encoded by the pufM gene. This study determined top-down controlled growth rates of total AAPB and subgroups of AAPB, as well as growth rates of total prokaryotes and total bacteria. Amplicon libraries of the pufM gene from a 2016 GoM mesocosm experiment and a freshwater sample were used to design new qPCR primers to target Rba and Rsb AAPB subgroups. Two manipulation experiments were conducted where grazers were reduced, one in Fall 2020 and one in Spring 2021 in brackish oligotrophic waters of Santa Rosa Sound, Pensacola, FL, USA. Apparent growth rates (AGR), estimated growth rates, and abundance of AAPBs and subgroups varied seasonally and by subgroups. In spring, total AAPB abundance was significantly higher in grazer reduced samples than the control. Relative abundance of denovo 0 was significantly greater in the control treatment than in the grazer reduced treatment. During fall and spring, total AAPBs and subgroups Rsb 7 and 9 had higher AGRs and estimated growth rates in the grazer reduced treatments. In fall grazer reduced treatments, AAPB subgroups denovo 0 and 35 had AGRs of 0.35 day-1 and 0.08 day-1, respectively. During spring, denovo 0 and 35 had a negative AGR. Despite differences in growth rates of AAPB subgroups, in fall and spring, total AAPBs had increased AGRs 0.55 day-1 and 1.22 day-1, respectively.
Details
- Title
- Regrowth of Subtropical Aerobic Anoxygenic Photoheterotrophic Bacteria Upon Grazer Removal
- Resource Type
- Thesis
- Contributors
- Lisa L Waidner (Advisor)Joe E Lepo (Committee Member)Jane Caffrey (Committee Member)
- Publisher
- University of West Florida Libraries
- Format
- pdf
- Number of pages
- 125
- Copyright
- Copyright © 1996 by Elizabeth D. Vickers All rights reserved
- Identifiers
- 99380092797006600
- Academic Unit
- Biology; Center for Environmental Diagnostics and Bioremediation
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida