ROLE OF SALICYLIC ACID AND ABSCISIC ACID IN THE DEFENSE RESPONSE OF DIOSCOREA BULBIFERA L. TO FUNGAL PATHOGENS
Alexander Luis Penabade
University of West Florida
Master of Science (MS), University of West Florida
2014
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Abstract
Plant-microbe interactions occur frequently in nature and can be detrimental to survival of the plant. In order for plants to survive these negative interactions with microbes the plant must recognize and appropriately respond to the attacking pathogen. In the response to pathogen attack signaling molecules are induced to initiate expression of pathogenesis-related proteins. These signaling molecules interact to form a fine tuned defense response to combat different pathogens in different ways. The defense response in invasive plants has not been well studied. This study focuses on the response to fungal pathogens in an invasive plant (Dioscorea bulbifera L.). Here, two classes of pathogenesis related proteins, PR-2 and PR-3, which are comprised of glucanases and chitinases, were used to monitor the defense response after treatments with fungal elicitor or the signaling molecules salicylic acid and abscisic acid. This study provides evidence that the reproductive tissue has constitutive expression of these pathogenesis related proteins whereas leaves (vegetative tissue) exhibit an induced response to pathogen attack. Further investigation into D. bulbifera L. may elucidate which signaling pathway is involved with the constitutive defense response in reproductive tissue, as well as, the response to fungal pathogens in leaves.