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Thesis
PHOSPHATIDYLINOSITOL SYNTHASE OF TETRAHYMENA
University of West Florida
Master of Science (MS), University of West Florida
2006
Abstract
Phosphatidylinositol synthase [CDP-diacylglycerol: myo-inositol 3-phosphatidyltransferase, E.C. 2.7.8.11] is present in all eukaryotic cells and some bacterial cells. Predominantly found on the cytoplasmic side of the endoplasmic reticulum, this bifunctional enzyme catalyzes the de novo synthesis of phosphatidylinositol from cytidine diphosphate-diacylglycerol and myo-inositol. The enzyme also catalyzes a headgroup exchange reaction wherein the inositol headgroup of phosphatidylinositol is exchanged for a different molecule of inositol. Headgroup exchange activity associated with phosphatidylinositol synthase from the protist Tetrahymena was investigated to establish whether isomers of inositol other than myo-inositol are suitable substrates. This investigation revealed that eight of the nine isomers of inositol examined were used as substrates in the headgroup exchange reaction. Furthermore, cytidine monophosphate was shown to be necessary for maximal headgroup exchange activity. These data provide evidence for a mechanism by which novel phosphatidyl-non-myo-inositols can be formed in vivo.
Details
- Title
- PHOSPHATIDYLINOSITOL SYNTHASE OF TETRAHYMENA
- Resource Type
- Thesis
- Publisher
- University of West Florida
- Format
- text
- Identifiers
- 99380090863906600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida