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Thesis
PATHOGENESIS RELATED PROTEINS IN DIOSCOREA BULBIFERA L.
University of West Florida
Master of Science (MS), University of West Florida
2014
Abstract
Plants experience a myriad of diverse stress stimuli, and because they are sessile organisms they have had to evolve intricate defense mechanisms for survival. Defenses include a unique family of proteins called pathogenesis related (PR) proteins. These low molecular weight proteins can be constitutively expressed or highly inducible upon elicitation by fungal pathogens. The signaling pathways that lead to the expression of the PR proteins are regulated by the plant hormones salicylic acid (SA), jasmonic acid (JA), and abscisic acid (ABA). Western blot data provide evidence that the PR proteins chitinase and a-1,3-glucanase are present in both leaf and tuber tissues of Dioscorea bulbifera L. Upon treatment of leaf tissue with SA, expression of a 47 kDa chitinase was up regulated after both 24 and 48 hours. Leaves treated with Fusarium graminearum cell wall fragments exhibited a unique triplet of bands with molecular weights of 28, 24, and 16 kDa. Tuber tissues exhibited constitutive expression of a 119 kDa and a 34 kDa chitinase and a 31 kDa a-1,3-glucanase across all treatments. This thesis provides evidence that supports the role of chitinases and a-1,3-glucanases as part of a defense response in aerial tubers and leaves of D. bulbifera L.
Details
- Title
- PATHOGENESIS RELATED PROTEINS IN DIOSCOREA BULBIFERA L.
- Resource Type
- Thesis
- Publisher
- University of West Florida
- Format
- text
- Identifiers
- 99380090852406600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida