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Thesis
KETAMINE INCREASES SUSCEPTIBILITY TO INFLUENZA IN MICE
University of West Florida
Master of Science (MS), University of West Florida
2013
Abstract
Influenza is a constant threat to public health. For this reason, many research hours and dollars have been invested in understanding the virus and how to better protect humans from the sometimes-deadly effects of influenza. BALB/c and C57Bl/6 mice have been common models for studying influenza, and ketamine anesthetization has been commonly used when inoculating mice with influenza. Recent literature indicates that ketamine may be affecting the immune response by interfering with the functional maturation of dendritic cells and other innate immune mediators. This study shows that ketamine increases susceptibility to the H1N1 influenza virus, A/PR/8/34. Most of the effect is shown to be due to the presence of ketamine independent of anesthetization. In this study, TPCK-trypsin parameters were also evaluated in MDCKpropagated influenza. Replenishing trypsin 24 hours post-infection increased the viral titer. MDCK-propagated influenza was also tested for infectivity in multiple cells lines, MDCK, NF-1, Sp2/0-Ag14, and JAWSII. Only MDCK and NF-1 showed influenza growth 48 hours after infection.
Details
- Title
- KETAMINE INCREASES SUSCEPTIBILITY TO INFLUENZA IN MICE
- Resource Type
- Thesis
- Publisher
- University of West Florida
- Format
- text
- Identifiers
- 99380090865306600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida