Thesis
Early Biomarker Detection In Alzheimer's Disease Using Post Translational Modifications Of The Tau Protein
University of West Florida Libraries
Master of Science (MS), University of West Florida
2023
Abstract
Alzheimer’s disease (AD) is a devastating brain disorder that affects more than 35 million people and their families globally which always ends in death (Shui et al. 2018; Weller and Budson 2018). Cognitive impairments like progressive memory loss and personality change are some of the common and notable symptoms of AD (Shui et al. 2018). With no treatment it is projected that the number of people affected by AD will double every 20 years to more than 115.4 million by the year 2050 (Shui et al. 2018; Weller and Budson 2018). Among the challenges to find a treatment for AD is reliable diagnostics. Therefore, a major research emphasis is the identification of specific and selective biomarkers that are associated with disease progression that do not rely solely on clinical impression. The present study includes the use of phage display to find such markers, using post translational changes of tau identified in cerebrospinal fluid as the target. Using an iterative panning procedure to assess human CSF, phages were found within a peptide-library that detected a SUMO or glycosylation dependent conformations of tau. In some cases, phages were more selective to AD-associate PTM changes, while in others control-associated changes were more readily detected. The added use of a phage-based assay would be a significant improvement to determine differences between AD and control patients, adding confidence in a diagnosis.
Details
- Title
- Early Biomarker Detection In Alzheimer's Disease Using Post Translational Modifications Of The Tau Protein
- Resource Type
- Thesis
- Contributors
- Rodney P Guttmann (Committee Chair)Peter Cavnar (Committee Member)Scott Taylor (Committee Member)
- Publisher
- University of West Florida Libraries
- Format
- pdf
- Number of pages
- 47
- Copyright
- Permission granted to the University of West Florida Libraries by the author to digitize and/or display this information for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires the permission of the copyright holder.
- Identifiers
- 99380461596206600
- Academic Unit
- Biology; Hal Marcus College of Science and Engineering
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida