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Thesis
Detection of Phosphoinositide-Binding Proteins in Tetrahymena Vorax Using Liposomes as an Affinity Matrix
University of West Florida Libraries
Master of Science (MS), University of West Florida
2011
Abstract
The methodology developed by Knodler and Mayinger (2005) was modified to detect phosphoinositide-binding proteins in Tetrahymena vorax using liposomes as an affinity matrix. Various protein samples were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis. Gels were stained for total proteins with SYPRORuby stain in order to digitally quantify the presence of phosphoinositide-binding proteins. Qualitative analysis of the gels after total protein staining was also utilized to detect the presence of phosphoinositide-binding proteins. Five proteins with molecular weights of 58, 49, 44, 31 and 23 kDa were detected that bound to phosphatidylinositol 4- phosphate, and seven proteins with molecular weights of 51, 48, 34, 32, 20, 19 and 16 were detected that bound to phosphatidylinositol 3-phosphate. The adapted methodology was useful to an extent in the detection of phosphoinositide-binding proteins in T. vorax.
Details
- Title
- Detection of Phosphoinositide-Binding Proteins in Tetrahymena Vorax Using Liposomes as an Affinity Matrix
- Resource Type
- Thesis
- Publisher
- University of West Florida Libraries
- Format
- pdf
- Number of pages
- 47
- Copyright
- © 2022 Taylor N. Walkup
- Identifiers
- 99380090718006600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida