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DETECTION OF THE ESV-1 VIRUS AND SPECIES DETERMINATION FOR ECTOCARPUS, ESPECIALLY FROM THE NORTHERN GULF OF MEXICO
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DETECTION OF THE ESV-1 VIRUS AND SPECIES DETERMINATION FOR ECTOCARPUS, ESPECIALLY FROM THE NORTHERN GULF OF MEXICO

Christopher Robin Main
University of West Florida
Master of Science (MS), University of West Florida
2008

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Abstract

Previous investigators estimated the EsV-1 virus infected nearly 50% of the worldwide populations of Ectocarpus siliculosus. In the present study, E. siliculosus was collected from the northern Gulf of Mexico, purchased from culture collections, and obtained from a previous investigator. The virus was detected using DNA extracts and a probe for GP-1 from the protein coat, followed by PCR and electrophoresis techniques and gene sequencing. In addition, all samples of Ectocarpus were observed microscopically and cell dimensions measured for comparison with cultures of published known species. ANOVA was applied to microscopic data. Alternatively, sequences for both 18S rRNA and Ribulose 1,5-bisphosphate carboxylase/oxygenase (RUBISCO) large subunit (rbcL) were used to separate species. Gene sequence alignments were made using BioEdit and analyzed with the PAUP* software package. While phylogenetic trees were somewhat confounding and microscopic data was limited, it appeared that all collected and purchased samples were E. siliculosus, even one previously identified as E. fasciculatus. None of the specimens from the Gulf of Mexico tested positive for the EsV-1 virus; however, for the first time, three samples from established culture collections were reported as infected.
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