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Thesis
Characterizing the effects of atypical antipsychotics on the neutrophil model cell line, PLB-985
University of West Florida,
Master of Science (MS), University of West Florida
2021
Abstract
Atypical antipsychotics (AAPs) are a class of drug used to treat several mental diseases like bipolar disorder, obsessive-compulsive disorder, and schizophrenia. However, these substances are also known to cause a rare, yet severe and sometimes fatal syndrome known as neutropenia. Neutropenia (and its more acute form, agranulocytosis) is characterized by a significant decrease in an individual's circulating neutrophils--the most abundant white blood cell. Neutrophils are essential for proper innate immunity, and depletion can result in higher risk of developing life-threatening illnesses. How atypical antipsychotics induce neutropenia is not well-understood, though research is ongoing. In this study, we aim to characterize the effects of four AAPs--clozapine, olanzapine, quetiapine and aripiprazole--on PLB-985 cell viability, as assessed by the XTT Cell Viability Assay. We found that only aripiprazole is able to significantly decrease dPLB-985 cell viability after 48-hour treatment. We hypothesized that aripiprazole's negative effect on cell viability is through its unique mechanism of action as a partial dopamine agonist; however, our results suggest that aripiprazole's effects are independent of dopamine receptor agonism.
Details
- Title
- Characterizing the effects of atypical antipsychotics on the neutrophil model cell line, PLB-985
- Resource Type
- Thesis
- Publisher
- University of West Florida,; [Pensacola, Florida] :
- Format
- text;electronic resource;remote;computer;online resource
- Number of pages
- 1 online resource (vi, 34 leaves : illustrations)
- Copyright
- http://rightsstatements.org/vocab/InC-EDU/1.0/
- Identifiers
- 99380090733506600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida