Metrics
6 File views/ downloads
34 Record Views
Thesis
CYTOKINE AND AUTOPHAGY PROTEIN PRODUCTION IN NEUTROPHILS FOLLOWING VIGOROUS EXERCISE IN HOT AND COLD ENVIRONMENTS
University of West Florida
Master of Science (MS), University of West Florida
2015
Abstract
This study tested the hypothesis that high-intensity endurance exercise would induce synthesis of heat shock protein, cytokine, and autophagy proteins in the human neutrophil. Nine healthy, recreationally-active males (ages 20-24) performed 30 min of vigorous (70% of VO2max) cycling exercise for two separate testing sessions (hot vs. cold) in an environmental chamber. Blood samples were drawn on three separate time points (pre-exercise, post-exercise, and one hour post-exercise). Neutrophils were isolated from the whole blood samples, treated and prepared for western blotting analysis. Heat shock protein 70kDa, NF-kB transcription factor, TNF-alpha, LC3, and Ubiquitin proteins were quantified by western blotting. Friedman's test was performed on levels of HSP-70, NF-kB, LC-3, TNF-alpha, and Ub, and no significance was established (p = 0.725, p = 0.654, p = 0.497, p = 0.098, and p = 0.825, respectively). These findings may suggest that vigorous exercise is warranted in these environments without inducing unnecessary production of neutrophil mediated inflammation.
Details
- Title
- CYTOKINE AND AUTOPHAGY PROTEIN PRODUCTION IN NEUTROPHILS FOLLOWING VIGOROUS EXERCISE IN HOT AND COLD ENVIRONMENTS
- Resource Type
- Thesis
- Publisher
- University of West Florida
- Format
- text
- Copyright
- http://rightsstatements.org/vocab/InC-EDU/1.0/
- Identifiers
- 99380090722506600
- Academic Unit
- Usha Kundu, MD College of Health
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida