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Thesis
Biodetoxification of Aristolochia serpentaria by bacteria: Comparison of Liquid-submerged Fermentation with Solid-state Fermentation
University of West Florida Libraries
Master of Science (MS), University of West Florida
2024
Abstract
Aristolochic acids (AAs) are a naturally occurring class of nitrophenanthrene carboxylic acids found in several therapeutic herbs of the Aristolochia genus that can be carcinogenic and genotoxic and cause irreversible liver and kidney damage in humans. Like other secondary metabolites in plants including terpenoids, steroids and phenolic compounds, AA can be extracted from the Aristolochia plants' roots, stems and leaves where AA1 and AA2 are the primary forms found. Although most countries have already banned the clinical use of AA-containing traditional Chinese medicines (TCMs), they have not been entirely eliminated because of their high perceived pharmacological value.
Extraction techniques that have been used for removing toxic compounds in traditional herbal medicines, including liquid-liquid extraction (LLE) and solid-phase extraction (SPE), are expensive. There has not been much research on the microbial detoxification of Aristolochia plants except for a recent study that involved the use of an endophytic fungi, Neocosmospora solani, on Asarum sp. Hence, there is a need for research on biodetoxification using bacterial communities.
This research explored the use of fermentation as a processing technique to selectively remove the cancer-causing compound (aristolochic acids) in Aristolochia serpentaria leaves, hence reducing toxicity to enable their continued use in traditional medicines. The removal of AA1 and AA2 from leaf tissues by Pseudomonas multiresinovorans can be greatly influenced by incubation temperature and inoculum sizes while tolerating a wide range of temperatures. Other factors that can determine the rate of AA removal are the type of treatments leaves are subjected to before detoxification and lastly the type of fermentation used.
Details
- Title
- Biodetoxification of Aristolochia serpentaria by bacteria
- Resource Type
- Thesis
- Contributors
- Jim C. Spain (Committee Chair)Lisa Waidner (Committee Member)Wade Jeffrey (Committee Member)
- Publisher
- University of West Florida Libraries
- Format
- pdf
- Number of pages
- 51
- Copyright
- © 2024 Damilola Balogun Olubunmi
- Identifiers
- 99380599442406600
- Academic Unit
- Biology
- Language
- English
- Awarding Institution
- University of West Florida; Master of Science (MS)
- Theses and Dissertations
- Master of Science (MS), University of West Florida