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Poster
Optimizing Primers for DNA Extraction of nifH - Do Epiphytes Play a Role in N Fixation?
University of West Florida Libraries
Student Scholar Symposium & Faculty Research Showcase (University of West Florida, Pensacola, Florida, 04/20/2023)
04/20/2023
Abstract
The Gamma primer showed nonspecific primer annealing, the literature information will be checked in order to find out if the correct sequence was used to predict optimal parameters. With the correct parameters for the CYB primer, this one will proceed to be used with the samples of Thalassia testudinum and Halodule wrightii from Santa Rosa Sound to continue the study on nitrogen fixation by epiphytes. The next steps are to finish DNA extraction of epiphytes from the seagrass blades collected and use these primers to identify the presence of the nifH genes.
Details
- Title
- Optimizing Primers for DNA Extraction of nifH - Do Epiphytes Play a Role in N Fixation?
- Resource Type
- Poster
- Event
- Student Scholar Symposium & Faculty Research Showcase (University of West Florida, Pensacola, Florida, 04/20/2023)
- Contributors
- Jane M Caffrey (Faculty Mentor) - University of West Florida, Hal Marcus College of Science and EngineeringLisa A Waidner (Faculty Mentor) - University of West Florida, Biology
- Publisher
- University of West Florida Libraries; Argo Scholar Commons
- Format
- pdf
- Copyright
- Permission granted to the University of West Florida Libraries by the author to digitize and/or display this information for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires the permission of the copyright holder.
- Identifiers
- 99380475896506600
- Academic Unit
- Biology; Center for Environmental Diagnostics and Bioremediation ; 2023 Student Scholars Symposium and Faculty Research Showcase; Office of Undergraduate Research; Hal Marcus College of Science and Engineering
- Language
- English