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Poster
Assessing neutrophil viability after atypical antipsychotic exposure using cell line PLB-985
University of West Florida Libraries
Student Scholar Symposium & Faculty Research Showcase (University of West Florida, Pensacola, Florida, 2021)
2021
Abstract
Neutrophils comprise 60-70% of an individual's white blood cells and are an essential part of our innate immune systems. Neutrophils act as first-responders to sites of injury or potential infection, where they help to initiate inflammatory responses and/or aid in destruction of an infectious agent. (Witko-Sarsat, 2000)
Atypical antipsychotics, a class of drug commonly used to treat disorders like schizophrenia and insomnia, are known to cause neutropenia and agranulocytosis (a depletion of circulating neutrophils) as a rare, but severe, side effect. (Hong & Wong, 2001) However, the mechanism(s) by which these drugs induce neutropenia is still in question.
In this study, we used the neutrophil model cell line, PLB-985, to assess the effects of four atypical antipsychotics (clozapine, olanzapine, aripiprazole and quetiapine) on cell viability using a plate-based, colorimetric bioassay.
After collecting this initial data, we hypothesize that the reduction in cell viability seen after 48-hour exposure to 20μM and 50μM concentrations of aripiprazole is due to its unique mechanism of action as a partial dopamine agonist. Therefore, we seek to determine if we can both rescue the reduction of cell viability by introducing dopamine antagonists, as well as replicate the phenotype by exposing cells to dopamine hydroch
Details
- Title
- Assessing neutrophil viability after atypical antipsychotic exposure using cell line PLB-985
- Resource Type
- Poster
- Event
- Student Scholar Symposium & Faculty Research Showcase (University of West Florida, Pensacola, Florida, 2021)
- Contributors
- Dr. Peter Cavnar (Faculty Mentor)
- Publisher
- University of West Florida Libraries; Argo Scholar Commons
- Format
- pdf
- Copyright
- Permission granted to the University of West Florida Libraries to digitize and/or display this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires the permission of the copyright holder.
- Identifiers
- 99380090777506600
- Academic Unit
- Office of Undergraduate Research; 2021 Student Scholars Symposium and Faculty Research Showcase
- Language
- English