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Book chapter
Presence of Antibody-Dependent Cellular Cytotoxicity against SARS-CoV-2 in COVID-19 Plasma
Advances in Clinical Immunology, Medical Microbiology, COVID-19, and Big Data, pp.777-790
Jenny Stanford Publishing, 1
2022
Abstract
The coronavirus disease of 2019 (COVID-19) pandemic is caused by the novel SARS-CoV-2 virus. Antibody responses develop against SARS-CoV-2 during the infection in many subjects tend to increase over the course of disease and correlate with viral RNA titer. HEK-293T cells were cultured in Dulbecco’s modified Eagle medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. Cells expressing either the SARS-CoV-2 spike or nucleocapsid proteins were generated for use in the immunofluorescence assay and as target cells in the ADCC assay. The major implication of our findings is that efficacy of COVID-19 vaccine candidates should not be evaluated solely based on the level of nAb elicited, but rather the totality of SARS-CoV-2 specific humoral responses elicited.
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Details
- Title
- Presence of Antibody-Dependent Cellular Cytotoxicity against SARS-CoV-2 in COVID-19 Plasma
- Edition
- 1
- Publication Details
- Advances in Clinical Immunology, Medical Microbiology, COVID-19, and Big Data, pp.777-790
- Resource Type
- Book chapter
- Publisher
- Jenny Stanford Publishing
- Number of pages
- 14
- Copyright
- © Taylor & Francis
- Identifiers
- 99380577556806600
- Academic Unit
- Biology; Hal Marcus College of Science and Engineering
- Language
- English