Metrics
89 Record Views
Book chapter
Measurement of UVB-induced DNA damage in marine planktonic communities
Methods in Microbiology, pp.469-485
Elsevier Ltd
2001
Web of Science ID: WOS:000169406600023
Abstract
Ultraviolet radiation (UVR) has been recognized for many years as a potential stress for organisms in a variety of environments. Decrease in phytoplankton production may result in a decline in bacterial production, which may be compounded by direct UVB effects on bacterioplankton. UVR may directly affect viruses, bacteria, phytoplankton, or zooplankton via direct DNA damage and reduced rates of production. The effects of UVR on marine bacterioplankton are investigated by using radiolabeled precursor molecules such as 3H thymidine, and 3H- or 14C-leucine. It has been shown that bacterioplankton experience significant amounts of DNA damage (CPDs) in surface waters, often twice the amount of larger eukaryotic cells. Bacterioplankton accumulate DNA damage over a solar day. DNA damage extend to depths of 10 m or more in calm waters but the amount of damage may be significantly altered by surface water mixing events. Radioimmunoassay (RIA) technique is used for the measurement of specific DNA photoproducts in the DNA of UV-irradiated cells. DNA damage results are reported per unit (megabase) DNA, and are therefore independent of the concentration of DNA present in the original sample or the amount of DNA assayed. The sensitivity of the RIA is determined by the affinity of the antibody and specific activity of the radiolabeled antigen (probe).
Related links
Details
- Title
- Measurement of UVB-induced DNA damage in marine planktonic communities
- Publication Details
- Methods in Microbiology, pp.469-485
- Resource Type
- Book chapter
- Publisher
- Elsevier Ltd
- Copyright
- © 2001 Published by Elsevier Ltd.
- Identifiers
- WOS:000169406600023; 99380468034806600
- Academic Unit
- Center for Environmental Diagnostics and Bioremediation ; Hal Marcus College of Science and Engineering
- Language
- English