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Maintenance of myonuclear domain during mechanical ventilation induced diaphragmatic atrophy
Abstract   Peer reviewed

Maintenance of myonuclear domain during mechanical ventilation induced diaphragmatic atrophy

Joseph Matthew McClung, Andreas N Kavazis, Keith DeRuisseau, Darin Falk, Melissa Deering, Youngil Lee and Scott Powers
The FASEB journal, Vol.20(5), p.LB32
03/2006
DOI: https://doi.org/10.1096/fasebj.20.5.LB32

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Abstract

Unloading the diaphragm via mechanical ventilation (MV) results in rapid diaphragmatic fiber atrophy. We tested the hypothesis that MV‐induced diaphragmatic atrophy is associated with a loss of myonuclei via an apoptotic‐like mechanism resulting in a constant myonuclear domain. To test this postulate, Sprague‐Dawley rats were randomly assigned to one of three experimental groups: control, 6‐, or 12‐hour MV. Following 12‐hours of MV, Type I and Type IIa diaphragm myofiber cross sectional areas were decreased by 17% and 23%, respectively. Myonuclear content was also decreased after 12‐hours of MV and resulted in the maintenance of a constant myonuclear domain in all fiber types. Both 6‐ and 12‐hour MV resulted in significant increases in the number of terminal deoxynucleotidyl transferase (TdT)‐mediated dUTP nick‐end labeling (TUNEL) positive nuclei in diaphragm fibers. Collectively, these data support the concept that the myonuclear domain of diaphragm myofibers is maintained during MV‐induced atrophy and that myonuclear apoptosis contributes to this maintenance. This work was supported by National Institutes of Health (R01 HL62361).

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